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7900ht fast real-time pcr system  (Thermo Fisher)


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    Structured Review

    Thermo Fisher 7900ht fast real-time pcr system
    7900ht Fast Real Time Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/7900ht+fast+real+time+pcr+system/pm39757590-81-27-32
    Average 90 stars, based on 1 article reviews
    7900ht fast real-time pcr system - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Expressing:

    Article Title: The Effect of Hypoxia on Irisin Expression in HL-1 Cardiomyocytes
    Article Snippet: Reverse transcription reactions were performed using the High-Capacity cDNA Reverse Transcription Kit with RNase Inhibitor (Applied Biosystems, Waltham, MA, USA). .. The expression level of FNDC5 (FNDC5; TaqMan Gene Expression Assay Applied Biosystems; Mn01181543_1) was assessed using the 7900HT Fast Real-Time PCR System (Applied Biosystems). ..

    Article Title: Interleukin-10 contrasts inflammatory synaptopathy and central neurodegenerative damage in multiple sclerosis
    Article Snippet: Next, 400–500 ng of total RNA was reverse-transcribed using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystem) according to the manufacturer’s instructions. .. About 20 ng of cDNA was amplified with SensiMix II Probe Kit (Bioline, Meridian Life Science) in triplicate using the Applied Biosystem 7900HT Fast Real Time PCR system to evaluate the expression of Il10 (Mm01288386_m1) and Il1b (Mm00434228_m1) mRNAs. .. Data were represented relative to CFA samples by using the ΔΔCt method and β-actin (Actb, Mm00607939_s1) was used as internal control for the normalization.

    Article Title: Cold exposure accelerates lysine catabolism to promote cold acclimation via remodeling hepatic histone crotonylation.
    Article Snippet: Then 20 ng cDNA and 100 nmol of each primer were mixed with TB Green Premix Ex TaqII (Takara, RR820A). .. Reactions were performed in a 96-well plate using the 7900HT Fast Real Time PCR System (Applied Biosystems, Foster City CA), and the relative mRNA expression levels were determined according to our previously published work (Wu et al. 2023). ..

    Gene Expression:

    Article Title: The Effect of Hypoxia on Irisin Expression in HL-1 Cardiomyocytes
    Article Snippet: Reverse transcription reactions were performed using the High-Capacity cDNA Reverse Transcription Kit with RNase Inhibitor (Applied Biosystems, Waltham, MA, USA). .. The expression level of FNDC5 (FNDC5; TaqMan Gene Expression Assay Applied Biosystems; Mn01181543_1) was assessed using the 7900HT Fast Real-Time PCR System (Applied Biosystems). ..

    Real-time Polymerase Chain Reaction:

    Article Title: The Effect of Hypoxia on Irisin Expression in HL-1 Cardiomyocytes
    Article Snippet: Reverse transcription reactions were performed using the High-Capacity cDNA Reverse Transcription Kit with RNase Inhibitor (Applied Biosystems, Waltham, MA, USA). .. The expression level of FNDC5 (FNDC5; TaqMan Gene Expression Assay Applied Biosystems; Mn01181543_1) was assessed using the 7900HT Fast Real-Time PCR System (Applied Biosystems). ..

    Article Title: Interleukin-10 contrasts inflammatory synaptopathy and central neurodegenerative damage in multiple sclerosis
    Article Snippet: Next, 400–500 ng of total RNA was reverse-transcribed using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystem) according to the manufacturer’s instructions. .. About 20 ng of cDNA was amplified with SensiMix II Probe Kit (Bioline, Meridian Life Science) in triplicate using the Applied Biosystem 7900HT Fast Real Time PCR system to evaluate the expression of Il10 (Mm01288386_m1) and Il1b (Mm00434228_m1) mRNAs. .. Data were represented relative to CFA samples by using the ΔΔCt method and β-actin (Actb, Mm00607939_s1) was used as internal control for the normalization.

    Article Title: The mitochondrial orf125 affects male fertility of Solanum tuberosum (+) S. commersonii somatic hybrids and participates in the onset of “Tuberosum”-Type CMS and evolution of common potato
    Article Snippet: .. These reactions were run on the 7900HT Fast Real Time PCR system (Applied BioSystems, USA). ..

    Article Title: Parsing digital or analog TCR performance through piconewton forces
    Article Snippet: Once thawed, the tissues were homogenized by a tissue disruptor (QIAGEN), and RNA was extracted with a PureLink RNA Mini kit (Invitrogen). .. Reverse transcription was conducted with the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems), and quantitative PCR (qPCR) was performed with PowerUP SYBR Green Master Mix for qPCR (Applied Biosystems) and primers specific for NP (forward: 5′-GAT TGG TGG AAT TGG ACG; reverse: 5′-AGA GCA CCA TTC TCT CTA TT-3′) using the 7900HT Fast Real Time PCR System (Applied Biosystems). ..

    Article Title: Mechanisms of QingRe HuoXue Formula in atherosclerosis Treatment: An integrated approach using Bioinformatics, Machine Learning, and experimental validation.
    Article Snippet: .. With SYBR Green as the detection fluorophore, we performed qRT-PCR using an Applied Biosystems 7900HT Fast Real-Time PCR System. ..

    Article Title: Implications of high PsSAD expression and oleic acid content in defining the Sujata phenotype of Papaver somniferum
    Article Snippet: Papaver somniferum L. produces therapeutically-useful alkaloids like morphine, codeine, papaverine, and thebaine that accumulate in the latex of its capsule.. Apart from its alkaloids, the edible seeds of the plant have high nutritional value and culinary use, with the seed oil being rich in the health-promoting unsaturated fatty acids.. Sujata is low alkaloid-producing latex-less culinary variety of P. somniferum developed from an alkaloid-rich gum harvest parent (Sampada) for curtailing the narcotic menace of morphine and opium (dried latex).

    Article Title: Cold exposure accelerates lysine catabolism to promote cold acclimation via remodeling hepatic histone crotonylation.
    Article Snippet: Then 20 ng cDNA and 100 nmol of each primer were mixed with TB Green Premix Ex TaqII (Takara, RR820A). .. Reactions were performed in a 96-well plate using the 7900HT Fast Real Time PCR System (Applied Biosystems, Foster City CA), and the relative mRNA expression levels were determined according to our previously published work (Wu et al. 2023). ..

    Article Title: Regulatory effect of rapamycin on recruitment and function of myeloid-derived suppressor cells in heart failure.
    Article Snippet: .. The amplification and detection of these gene products were quantified on a 7900HT Fast Real-Time PCR System (Applied Biosystems, Foster City, CA) with 40 cycles of 15 s at 95 ◦C and 1 min at 60 ◦C. ..

    Amplification:

    Article Title: Interleukin-10 contrasts inflammatory synaptopathy and central neurodegenerative damage in multiple sclerosis
    Article Snippet: Next, 400–500 ng of total RNA was reverse-transcribed using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystem) according to the manufacturer’s instructions. .. About 20 ng of cDNA was amplified with SensiMix II Probe Kit (Bioline, Meridian Life Science) in triplicate using the Applied Biosystem 7900HT Fast Real Time PCR system to evaluate the expression of Il10 (Mm01288386_m1) and Il1b (Mm00434228_m1) mRNAs. .. Data were represented relative to CFA samples by using the ΔΔCt method and β-actin (Actb, Mm00607939_s1) was used as internal control for the normalization.

    Article Title: Regulatory effect of rapamycin on recruitment and function of myeloid-derived suppressor cells in heart failure.
    Article Snippet: .. The amplification and detection of these gene products were quantified on a 7900HT Fast Real-Time PCR System (Applied Biosystems, Foster City, CA) with 40 cycles of 15 s at 95 ◦C and 1 min at 60 ◦C. ..

    Reverse Transcription:

    Article Title: Parsing digital or analog TCR performance through piconewton forces
    Article Snippet: Once thawed, the tissues were homogenized by a tissue disruptor (QIAGEN), and RNA was extracted with a PureLink RNA Mini kit (Invitrogen). .. Reverse transcription was conducted with the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems), and quantitative PCR (qPCR) was performed with PowerUP SYBR Green Master Mix for qPCR (Applied Biosystems) and primers specific for NP (forward: 5′-GAT TGG TGG AAT TGG ACG; reverse: 5′-AGA GCA CCA TTC TCT CTA TT-3′) using the 7900HT Fast Real Time PCR System (Applied Biosystems). ..

    SYBR Green Assay:

    Article Title: Parsing digital or analog TCR performance through piconewton forces
    Article Snippet: Once thawed, the tissues were homogenized by a tissue disruptor (QIAGEN), and RNA was extracted with a PureLink RNA Mini kit (Invitrogen). .. Reverse transcription was conducted with the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems), and quantitative PCR (qPCR) was performed with PowerUP SYBR Green Master Mix for qPCR (Applied Biosystems) and primers specific for NP (forward: 5′-GAT TGG TGG AAT TGG ACG; reverse: 5′-AGA GCA CCA TTC TCT CTA TT-3′) using the 7900HT Fast Real Time PCR System (Applied Biosystems). ..

    Article Title: Mechanisms of QingRe HuoXue Formula in atherosclerosis Treatment: An integrated approach using Bioinformatics, Machine Learning, and experimental validation.
    Article Snippet: .. With SYBR Green as the detection fluorophore, we performed qRT-PCR using an Applied Biosystems 7900HT Fast Real-Time PCR System. ..

    Quantitative RT-PCR:

    Article Title: Mechanisms of QingRe HuoXue Formula in atherosclerosis Treatment: An integrated approach using Bioinformatics, Machine Learning, and experimental validation.
    Article Snippet: .. With SYBR Green as the detection fluorophore, we performed qRT-PCR using an Applied Biosystems 7900HT Fast Real-Time PCR System. ..

    Blocking Assay:

    Article Title: Implications of high PsSAD expression and oleic acid content in defining the Sujata phenotype of Papaver somniferum
    Article Snippet: Papaver somniferum L. produces therapeutically-useful alkaloids like morphine, codeine, papaverine, and thebaine that accumulate in the latex of its capsule.. Apart from its alkaloids, the edible seeds of the plant have high nutritional value and culinary use, with the seed oil being rich in the health-promoting unsaturated fatty acids.. Sujata is low alkaloid-producing latex-less culinary variety of P. somniferum developed from an alkaloid-rich gum harvest parent (Sampada) for curtailing the narcotic menace of morphine and opium (dried latex).



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